International Journal of Coronaviruses

International Journal of Coronaviruses

Current Issue Volume No: 3 Issue No: 3

Short-communication Article Open Access
  • Available online freely Peer Reviewed

  • Identify The Effects Of Ultra Weak Light On Alphacoronavirus And Vero Cells

    1 Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of Korea. 

    2 Department of Veterinary Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Vietnam. 

    3 Biolight corporation in Research Center, Seoul, Republic of Korea. 

    4 Noah Biotech Company in Research Center, Suwon, Republic of Korea. 

    Abstract

    Coronavirus is causing many diseases and economic pain to humans and animals. The present study demonstrated how ultra weak lightcause changes in porcine epidemic diarrhea virus (PEDV) and Vero cells.

    Ultra weak lightactivates Vero cells by lowering pH of maintain media, but have proven no effect of killing the virus.
    Author Contributions
    Received Aug 18, 2021     Accepted Aug 19, 2021     Published Aug 25, 2021

    Copyright© 2021 Chung Hee-Chun, et al.
    License
    Creative Commons License   This work is licensed under a Creative Commons Attribution 4.0 International License. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

    Competing interests

    The authors have declared that no competing interests exist.

    Funding Interests:

    Citation:

    Chung Hee-Chun, Giap Nguyen Van, Do Hai-Quynh, Park Mi-Jung, Su Yang Jeong et al. (2021) Identify The Effects Of Ultra Weak Light On Alphacoronavirus And Vero Cells International Journal of Coronaviruses. - 3(3):1-5
    DOI 10.14302/issn.2692-1537.ijcv-21-3934

    Introduction

    Introduction

    Coronavirus is not only a problem for people like COVID-19, it causes a range of diseases in farm animals, some of which can be serious and are a threat to the farming industry 3. Economically significant coronaviruses of farm animals include porcine epidemic diarrhea virus (PEDV) and bovine coronavirus (BCoV), which both result in diarrhea in young age groups animals 4. PEDV has been circulating in Asia for several decades. It was identified for the first time during 1980s in Japan and China 8. PEDV became a global issue, especially in 2013, with a high death rate in the USA 9 Many researchers are working hard to get rid of PEDV, which has been a problem up until now 10.

    In this study, the change was observed as a result of exposure of ultra weak light to cells and viruses respectively.

    Results

    Results and discussion Testing the Influence of PEDV on the Ultra Weak Light

    We demonstrated changes in PEDV titers during exposure times for ultra weak light.

    Following exposure to ultra weak light, the PEDV inhibition above the logarithmic reduction = log 0.1 (20.567%) from 10h to 72h was confirmed, but the statistical analysis was not significant (Figure 1). The effectiveness of disinfectant as a disinfectant announced a maximum reduced titer, in which the virus titer was reduced by at least 4 log10 25 was not met to be an effective dilution factor.

    Changes in PEDV titers according to ultra weak light exposure times     Testing the Influence of Vero Cells in Response to the Ultra Weak Light

    We examined how Vero cells changes following exposure to ultra weak light.

    After exposure to ultra weak light, the pH level maintained lower than the control group for 10 to 96 h, which enabled Vero cells, increasing the number of cells by 1.2 to 1.5 times more than the control group. In particular, differences due to larger effects were identified during light exposure for over 72 hours, indicating that the statistical analysis was also significant (Figure 2). The ultra weak light activated the cell by decreasing the pH of the growth medium.

    Variation of Vero cell and pH due to exposure to ultra weak light     Identification of PEDV Inhibition in Vero Cells in Reaction to Ultra Weak Light

    We found changes in PEDV in Vero cells exposed to ultra weak light.

    After exposure to ultra weak light, the virus inhibition of log reduction=0.1 (20.567%) from 10h to 48h was confirmed, but statistical analysis showed no significant effects of virus reduction (Figure 3).

    PEDV Inhibition in exposed ultra weak light within Vero Cells

    Conclusion

    Affiliations:
    Affiliations: